cfx connect real time fluorescence quantitative pcr system (Bio-Rad)
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Cfx Connect Real Time Fluorescence Quantitative Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 15377 article reviews
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Quantitative RT-PCR:Article Title: Bone marrow mesenchymal stem cells alleviate neurological dysfunction by reducing autophagy damage via downregulation of SYNPO2 in neonatal hypoxic–ischemic encephalopathy rats Article Snippet: Total RNA was extracted using triazole reagent (Takara, Japan), and then was reverse transcribed into cDNA using a PCR kit (Takara). .. RT-qPCR analysis was performed using a Article Title: Single-cell transcriptomics reveal oxidative phosphorylation and oxidative stress in the superior temporal plane of non-syndromic cleft lip and palate fetuses. Article Snippet: Aim: Non-syndromic cleft lip and palate (NSCLP) is a common congenital disability that causes morphological and functional abnormalities in the craniofacial region.. Many studies have focused on clinical treatments and surgical aspects, but there has Jo ur na l P re -p ro of Journal Pre-proof been limited investigation into the neural mechanisms in individuals with NSCLP.. Therefore, it remains unclear whether developmental abnormalities in the brains of NSCLP fetuses exist. Real-time Polymerase Chain Reaction:Article Title: Bone marrow mesenchymal stem cells alleviate neurological dysfunction by reducing autophagy damage via downregulation of SYNPO2 in neonatal hypoxic–ischemic encephalopathy rats Article Snippet: Total RNA was extracted using triazole reagent (Takara, Japan), and then was reverse transcribed into cDNA using a PCR kit (Takara). .. RT-qPCR analysis was performed using a Article Title: Bifidobacterium breve HH079 alleviates early-life antibiotic-exposed colon dysbiosis in mice by restoring the gut microbiota and gut barrier function. Article Snippet: Food & Article Title: Cloning, bioinformatics analysis, and expression of the ubiquitin 2 (ubq-2) gene from the dog roundworm Toxocara canis Article Snippet: .. Additionally, transcription levels of TcUBQ-2 at these eight stages were quantified using real-time quantitative PCR (qPCR) on a LightCycler 96 Article Title: Single-cell transcriptomics reveal oxidative phosphorylation and oxidative stress in the superior temporal plane of non-syndromic cleft lip and palate fetuses. Article Snippet: Aim: Non-syndromic cleft lip and palate (NSCLP) is a common congenital disability that causes morphological and functional abnormalities in the craniofacial region.. Many studies have focused on clinical treatments and surgical aspects, but there has Jo ur na l P re -p ro of Journal Pre-proof been limited investigation into the neural mechanisms in individuals with NSCLP.. Therefore, it remains unclear whether developmental abnormalities in the brains of NSCLP fetuses exist. Article Title: High glucose facilitates hepatocellular carcinoma cell proliferation and invasion via WTAP-mediated HK2 mRNA stability. Article Snippet: Hepatocellular carcinoma (HCC) is one of the most common cancers, and diabetes is a risk factor for hepatocarcinogenesis.. N6-methyladenosine (m6A) methyltransferase WT1-associated protein (WTAP) is highly expressed in HCC and contributes to tumor progression.. However, its role in high glucose-driven HCC progression remains unclear. Article Title: Duck β-defensin 10 inhibits Salmonella enterica by disrupting bacterial membrane integrity and its association with gut microbiota dynamics Article Snippet: .. The Article Title: Duck β-defensin 10 inhibits Salmonella enterica by disrupting bacterial membrane integrity and its association with gut microbiota dynamics. Article Snippet: .. The Article Title: Huafengdan Inhibits Glioblastoma Cell Growth and Mobility by Acting on PLAU and CAV1 Targets Article Snippet: Total cellular RNA was extracted according to the manufacturer’s instructions, and RNA concentration and purity were determined using a NanoDrop 2000 Micro Nucleic Acid Quantifier (Wuhan servicebio technology CO., Ltd., Wuhan, Hubei Province, China). .. RNA was reverse-transcribed to cDNA using the One-Tube One-Step Genomic DNA Removal and Reverse Transcription kit according to the manufacturer’s instructions. qPCR was subsequently performed using the QuantStudio 5 SYBR Green Assay:Article Title: High glucose facilitates hepatocellular carcinoma cell proliferation and invasion via WTAP-mediated HK2 mRNA stability. Article Snippet: Hepatocellular carcinoma (HCC) is one of the most common cancers, and diabetes is a risk factor for hepatocarcinogenesis.. N6-methyladenosine (m6A) methyltransferase WT1-associated protein (WTAP) is highly expressed in HCC and contributes to tumor progression.. However, its role in high glucose-driven HCC progression remains unclear. Article Title: Huafengdan Inhibits Glioblastoma Cell Growth and Mobility by Acting on PLAU and CAV1 Targets Article Snippet: Total cellular RNA was extracted according to the manufacturer’s instructions, and RNA concentration and purity were determined using a NanoDrop 2000 Micro Nucleic Acid Quantifier (Wuhan servicebio technology CO., Ltd., Wuhan, Hubei Province, China). .. RNA was reverse-transcribed to cDNA using the One-Tube One-Step Genomic DNA Removal and Reverse Transcription kit according to the manufacturer’s instructions. qPCR was subsequently performed using the QuantStudio 5 Reverse Transcription:Article Title: Huafengdan Inhibits Glioblastoma Cell Growth and Mobility by Acting on PLAU and CAV1 Targets Article Snippet: Total cellular RNA was extracted according to the manufacturer’s instructions, and RNA concentration and purity were determined using a NanoDrop 2000 Micro Nucleic Acid Quantifier (Wuhan servicebio technology CO., Ltd., Wuhan, Hubei Province, China). .. RNA was reverse-transcribed to cDNA using the One-Tube One-Step Genomic DNA Removal and Reverse Transcription kit according to the manufacturer’s instructions. qPCR was subsequently performed using the QuantStudio 5 |
