cfx96 real time fluorescent quantitative pcr detection system  (Bio-Rad)

Journal: Biology

Article Title: One-Pot LAMP-Coupled CRISPR/Cas12b Assay Enables Sensitive Detection of Helicobacter pylori

doi: 10.3390/biology15100797

Figure Lengend Snippet: LAMP Primer Panel Screening. ( a ) Amplification curves for real-time LAMP; ( b ) agarose gel electrophoresis. CagA2/4/7: Positive controls for the 2nd, 4th, and 7th sets of CagA LAMP primers, respectively; NTC-CagA2/4/7: Negative controls for the 2nd, 4th, and 7th sets of CagA LAMP primers, respectively.

Article Snippet: The key instruments applied in this investigation included a −80 °C ultra-low temperature freezer (Aucma, Qingdao, China), a high-speed microcentrifuge (Michael Laboratory Instrument, Changsha, China), a microspectrophotometer (Xinling Biotechnology, Shanghai, China), micropipettes (Eppendorf, Hamburg, Germany), a biosafety cabinet (Xinbeixi Biotechnology, Jinan, China), Real-time fluorescence quantitative PCR(qPCR) instrument (Bioer Technology, Hangzhou, China), an electronic balance (Puchun Instrument, Shanghai, China), an electrophoresis apparatus (Yixin Analytical Instrument, Shanghai, China), and a gel imaging system (Yixin Analytical Instrument, Shanghai, China).

Techniques: Amplification, Agarose Gel Electrophoresis